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KMID : 0545120140240030427
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 3 p.427 ~ p.430
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Development of a Novel Immunochromatographic Assay for Rapid Detection of VanA Ligase-Producing Vancomycin-Resistant Enterococci
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Ji Gil-Yong
Song Hyung-Geun
Son Bo-Ra
Hong Seung-Bok
Kim Jong-Wan
Shin Kyeong-Seob
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Abstract
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We developed a novel immunochromatographic assay (ICA) (EZ-Step VanA rapid kit; Dinona, Korea) for the detection of VanA ligase from vancomycin-resistant enterococci (VRE). Of eight monoclonal antibodies screened by ELISAs, the VanA ligase ICA constructed with 1H9 plus 3G11 showed the greatest reactivity. The detection limit of the kit was 6.3 × 106 CFU per test. Of 127 vancomycin-resistant microorganisms, 100 vanA VRE were positive in the VanA ligase ICA, and 27 non-vanA vancomycin-resistant isolates were negative. These results were consistent with those of the PCR analyses. Thus, our ICA is a reliable and easy-to-use immunological assay for detecting VanA-producing VRE in clinical laboratories.
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KEYWORD
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vancomycin resistance, VanA, Immunochromatographic Assay, rapid detection, Enterococcus
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